Distribution of allele frequencies of non-synonymous and synonymous germline and somatic variants in melanoma patients.

<p>(a) Allele frequencies of germline and somatic variants. The mean allele frequencies were: higher for germline variants (ns = 6.125, s = 7.412) than for the somatic variants, (ns = 2.685, s = 1.857). (b) Comparison of the ratios of non-synonymous to synonymous germline and somatic variants. Both were significantly different from the 2∶1 ratio expected by chance alone, but in opposite directions (somatic, binomial p = 1.15354×10⁻⁵; germline binomial p = 6.3953×10⁻¹²⁶).