Ser-573 influences phosphorylation of Akt/PKB.

<p><i>A</i>. HEK293 cells transiently expressing GFP-IRS2 or GFP-IRS2-S573A were stimulated with 50 ng/ml IGF-1 for the indicated time points. 40 µg of total protein was separated on 7.5% SDS gels and membranes were incubated with GFP antibody to ensure equal expression levels and with p-Thr-308 and p-Ser-473 antibody respectively. Corresponding Akt/PKB reblots are shown. <i>B</i>. Densitometric analyses of Akt/PKB phosphorylation. Black diamonds represent IRS-2 wild type, white squares IRS2-S573A mutant. Phosphorylation was normalized against total protein and IRS-2 wild type stimulated with IGF-1 for 5 min was set as 1 (mean ± SEM; n = 3; *p<0.05 IRS-2 120 min vs. S573A 120 min; IRS-2 240 min vs. S573A 240 min).