Immunocytochemical analysis of endothelin receptor expression in primary rat retinal ganglion cells (RGCs).

<p>Primary rat RGCs were isolated from post-natal day 3–7 rat pups and immunocytochemical analysis of (<b>A</b>) ET_B and (<b>B</b>) ET_A expression was performed using a custom-made ET_B antibody and a commercially available ET_A antibody. The immunostaining was detected using corresponding Alexa 647 conjugated secondary antibodies. (<b>C</b>) A negative control immunostaining (Blank) in which the primary antibody was omitted showed minimal staining. Cells were counterstained with DAPI to detect cell nuclei. Scale bar indicates 20 µm.