<p>Lanes 1–4: non-induced, IPTG-induced <i>E. coli</i>, purified <i>Spli</i>Lec (CBB-stained), purified <i>Spli</i>Lec (silver-stained). Lane M: broad-range protein marker. Molecular weight of the marker is indicated as KDa.</p
<p>The expression of His-tagged rCRM197 was induced by IPTG. Molecular weight markers (M) are indica...
<p>(A) Expression products from <i>E. coli</i> BL21 transformed with recombinant SjTGR-pET41a and in...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lanes 1–3: control, induced and purified <i>Spli</i>Lec. Lanes 4–6: Western blotting using Click-...
<p>(A) The protein was induced with IPTG (0.5 mM) at 22°C for 16 h in E. coli BL21(DE3) cells, and a...
<p>Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induct...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>Lanes: M – MW markers, a - transformed <i>E. coli</i> BL21, b - transformed <i>E. coli</i> BL21 i...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Lane 1, protein marker; lane 2, lysate of <i>E. coli</i> transformed with pET32a-<i>EsLecF</i> wi...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>M: protein molecular weight marker; 1: protein lysates from recombinant <i>E</i>. <i>coli</i> bef...
<p>Lane 1, protein marker; lane 2, IPTG-induced recombinant EtPDIL protein at 0 h; lane 3, IPTG-indu...
<p>The purity of the proteins was analysed by SDS-PAGE using 10% (w/v) gel showing <b>(A)</b> PL1A (...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p>The expression of His-tagged rCRM197 was induced by IPTG. Molecular weight markers (M) are indica...
<p>(A) Expression products from <i>E. coli</i> BL21 transformed with recombinant SjTGR-pET41a and in...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
<p>Lanes 1–3: control, induced and purified <i>Spli</i>Lec. Lanes 4–6: Western blotting using Click-...
<p>(A) The protein was induced with IPTG (0.5 mM) at 22°C for 16 h in E. coli BL21(DE3) cells, and a...
<p>Lane M, protein molecular marker; Lane 1, before induction with 0.7 mM IPTG; Lane 2, after induct...
<p>SDS-PAGE analysis of the purified recombinant Cen219. M, marker proteins; lane 1, extracts of IPT...
<p>Lanes: M – MW markers, a - transformed <i>E. coli</i> BL21, b - transformed <i>E. coli</i> BL21 i...
<p>Lane 1, molecular mass standards. Lane 2, total protein of <i>E</i>. <i>coli</i> BL21 (DE3) pLysS...
<p>Lane 1, protein marker; lane 2, lysate of <i>E. coli</i> transformed with pET32a-<i>EsLecF</i> wi...
<p>Lanes: 1, protein molecular weight standard; 2, crude extract of un-induced cells; 3, crude extra...
<p>M: protein molecular weight marker; 1: protein lysates from recombinant <i>E</i>. <i>coli</i> bef...
<p>Lane 1, protein marker; lane 2, IPTG-induced recombinant EtPDIL protein at 0 h; lane 3, IPTG-indu...
<p>The purity of the proteins was analysed by SDS-PAGE using 10% (w/v) gel showing <b>(A)</b> PL1A (...
<p>Lane M: protein molecular weight marker; Lane 1: total protein of <i>E. coli</i> BL21(DE3); Lane ...
<p>The expression of His-tagged rCRM197 was induced by IPTG. Molecular weight markers (M) are indica...
<p>(A) Expression products from <i>E. coli</i> BL21 transformed with recombinant SjTGR-pET41a and in...
<p>Lane M: protein MW markers; Lanes 1–2: IPTG induced <i>E. coli</i> pET30a-MANB; Lanes 3–5: Negati...
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