Expression of the BRE-eGFP reporter in the developing lung mirrors faithfully activation of the canonical BMP pathway.

<p>A) Kinetics of eGFP and Id1 mRNA expression levels during lung development analysed by Real-Time PCR. Values represent mean ± SEM of five individually analyzed animals. B) eGFP profile of enzymatically digested P1 lungs. The gates used to separate lung cells by sorting into eGFP^neg, eGFP^med and eGFP^high are indicated. C) Representative Western-blotting of proteins prepared from sorted populations with an anti-pSmad1/5/8 antibody. D) Quantitation of the pSmad1/5/8 relative to the eGFP^neg group.Values represent mean ± SEM of two independent experiments. E) Analysis of mRNA expression levels for eGFP, Smad6, Id1, Id3 and Id2in sorted cells. Values represent mean ± SEM of seven individually analyzed experiments. Groups were compared using one-way analysis of variance (ANOVA) with Bonferonis's post-hoc analysis. *P<0.05, **P<0.01 and ***P<0.001.