BMSC inhibited LPS induced activation of LX2 and upregulation of TLR4.

<p>(A) The effect of different dose of LPS on LX2 cells activation as the IL-8 and TGF-β secretion. All data are shown as mean ± SD from three independent experiments. * P<0.05. (B) Relative mRNA expression of TLR4 in LX2 cells and two HSCs in response to LPS. Data are depicted relative to expression in cells without LPS, which are assigned a value of 1. (C) RT-PCR analysis of the HSC activation marker α-SMA, Col-1 and TLR4 in the indicated treatments. (D) Western blot analysis of the HSC activation marker α-SMA, Col-1 and TLR4 in the indicated treatments. Expression levels were normalized with GAPDH.