Development of neutralizing antibody and ADCC titers in macaques inoculated with SIV_mac239Δ<i>nef</i>.

<p>Plasma collected from 10 animals at 0, 3, 5, 7, 13 or 15, and 21 weeks after inoculation with SIV_mac239Δ<i>nef</i> was evaluated for its capacity to neutralize SIV_mac239 (A) and to direct ADCC against SIV_mac239-infected cells (B). The loss of relative light units (RLU) indicates the loss of virus-infected cells during an 8-hour incubation in the presence of plasma and an NK cell line. Target cells infected by SHIV_SF162P3 served as a negative control for all ADCC assays (gray). Dashed lines indicate 50% activity. Neutralizing antibody titers were compared with 50% ADCC titers (C), and with AUC values for ADCC (D). An odds ratio (OR) for the probability of detecting neutralization per log₁₀ increase in 50% ADCC titer, or per 1 AUC unit increase in ADCC activity, was estimated by logistic regression.