Western blot analysis of KSHV miRNA targets.

<p>Protein levels of two KSHV miRNA targets, TP53INP1 and YWHAE, were analyzed by Western blot in absence (0 ng) or presence (800 ng) of miR-K12-11 expression vector. Shown are two independent transfections of miR-K12-11. YWHAE showed moderate downregulation at the protein level, while TP53INP1 levels were reduced by more than 50% in the presence of miR-K12-11. Actin expression served as loading control.