Antigen-specific donor CD8⁺ T cell expansion following vaccination.

<p>Frequency of CS-TCR CD8⁺ T cells (with specificity for the dominant CD8⁺ T cell epitope from <i>P. yoelii</i> circumsporozoite protein, <i>Py</i>CSP_280–288) within the draining lymph nodes (DLN) and spleen (SPL) seven days after adoptive transfer into congenic hosts and vaccination with a single s.c. injection of lipid core peptides (LCP), or pooled peptides (P1, P2, P3, P4; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040928#pone-0040928-t001" target="_blank"><i>Table 1</i></a>) and CpG-Alum, or i.m. injection with DNA vaccine encoding <i>Py</i>CSP (D7) (A); or seven days after the final of three similar vaccinations given at <u>3</u> week intervals (W7) (B). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040928#s3" target="_blank">Results</a> (mean and SEM) with mice tested individually are shown (A, SPL and DLN n = 6 mice pooled data from two repeat experiments; B, SPL n = 5 mice from a representative experiment) except for B, DLN which were pooled from five mice (mean shown). Statistical comparisons are made to a control group that received adoptively transferred cells but no vaccination (No Vacc Ctrl) or as indicated on the graph, using one-way ANOVA with Bonferroni's post-test.