<i>ZNF750</i> mRNA and protein expression in Ca²⁺ induction and in human skin.

<p>(A) QRT-PCR of <i>ZNF750</i> mRNA expression during Ca²⁺ induction in NHEK cells. <i>Filaggrin</i> mRNA levels serve as internal control. Error bars represent mean values±SD, N = 3. (B) QRT-PCR of <i>ZNF750</i> mRNA expression during Ca²⁺ induction in HaCaT cells. Error bars represent mean values±SD, N = 3. (C) ZNF750 protein levels during Ca²⁺ induction in HaCaT cells, analyzed by anti-ZNF750 antibodies against both N-terminal and C-terminal regions. (D) QRT-PCR of <i>ZNF750</i>, <i>KRT1</i> and <i>FLG</i> mRNA expression in NHEK cells that were treated with PMA. Error bars represent mean values±SD, N = 2.