Cytokine production following prime-boost vaccinations.

<p>Mice were vaccinated s.c. three times at three week intervals with lipid core peptides (LCP), or pooled peptides (P1, P2, P3, P4; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040928#pone-0040928-t001" target="_blank"><i>Table 1</i></a>) and CpG-Alum, or i.m. injection with DNA vaccine encoding <i>Py</i>CSP. Seven days following the final vaccination the splenocytes were harvested and restimulated for three days with peptides as indicated. Cytokine levels in the supernatant were then determined by Cytokine Bead Array. Asterisks indicate a significant increase in cytokine production when compared to the restimulation-equivalent no-vaccine control. Y (yes) or N (no) indicate statistically significant (p = <0.05) increase in cytokine production when compared to those observed in controls that had received the same vaccinations but were cultured without peptide or ConA (media only). Significance determined using one-way ANOVA with Bonferroni's post-test (n = 5).