Silencing PRMT5 or p44 expression inhibited the growth of prostate cancer cells.

<p>(<b>A</b>) The shRNA-mediated silencing of PRMT5 or p44 expression in prostate cancer cells. Western blot analysis of whole-cell lysates made from LNCaP cells infected with lentivirus expressing the non-target (NT) shRNA (lanes 1, 5), PRMT5 (lanes 2–4), or p44 (lane 6) shRNAs. The shRNA-resistant PRMT5 (lane 3) or PRMT5 R368A mutant (lane 4) was expressed in the PRMT5-expressing LNCaP cells. (<b>B</b>) Growth curves of prostate cancer cells expressing NT shRNA, PRMT5 shRNAs, p44 shRNA, PRMT5 shRNAs plus PRMT5, p44 shRNA plus p44, or PRMT5 shRNAs plus PRMT5mt.