Metabolic fates of dietary fatty acid and monoacylglycerol in iMGL mice small intestine.

<p>Metabolism studies were conducted in vivo by administration of radiolabeled lipids into mouse duodenum, and metabolites were assayed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043962#s4" target="_blank">Methods</a>. Metabolic fates of (A) dietary ¹⁴C-oleic acid and (B) dietary ³H-monoolein in iMGL mice small intestine. Data are presented as the % of each metabolite relative to total metabolites. Inset shows an enlarged scale for MG (WT; n = 10 (FA) or 5 (MG), iMGL; n = 16 (FA) or 9 (MG). (C) ¹⁴C oleate incorporation into TG (cpm/mg protein) (WT; n = 10, iMGL; n = 16). (D) ³H monoolein incorporation into TG (cpm/mg protein) (WT; n = 5, iMGL; n = 9). (E) Percent fatty acid (¹⁴C oleate) oxidation in iMGL mice small intestine (WT; n = 10, iMGL; n = 18). (F) Fecal fat analysis. Fecal fat content is presented as the % of stool weight (WT; n = 6, iMGL; n = 5). Data represent mean ± S.E. * <i>p</i><0.05 versus wild type littermates.