A temporal gene expression profiling of hESC differentiation.

<p><b>A</b>, Experimental representation of the time kinetic multilineage differentiation. <b>B</b>, An unsupervised hierarchical clustering analysis demonstrates that a temporal gene expression pattern changes during differentiation and the major changes were observed between days 12 to 14. Three independent biological experiments were performed for statistical analysis (p≤0.05). <b>C</b>, The dendrogams pattern of array cluster showed that a two major negative correlation for day 0 to 9 and 12 to 21 differentiation. <b>D</b>, The selected germ layer lineage specific BP (Enrichment p≤0.01) for up-regulated transcripts demonstrated that the numbers of genes are optimal between days 12 and 15. The figure represents (i) endoderm-specific, ii) ectoderm-specific and iii) mesoderm-specific BP. The x-axis represents the days of differentiation, and the y-axis represents number of transcripts.