Differential changes in mtDNA copy number and mitochondrial transcript levels.

<p>(A) Relative mtDNA copy number was measured by qRT-PCR in third instar larvae of <i>Drosophila</i> lines expressing mutant <i>d-mtDNA helicase</i>. (B) Total DNA (10 µg) was fractionated in an 0.8% agarose gel, blotted to nylon membrane, and hybridized with radiolabeled probes for the mtDNA to observe multiple deletions. (C) Relative mitochondrial mRNA levels of the <i>ND5</i> and <i>COX I</i> genes was measured by qRT-PCR. Data represent the mean ± s.d., *<i>P</i>≤0.05, **<i>P</i>≤0.005 as compared with wild-type (<i>w¹¹¹⁸</i>).