Neurosphere culture of CD133⁺ and CD133

<p>⁻<b>i-SOX2-DAOY Cells in the absence and presence of Dox.</b> i-SOX2-DAOY cells were FACS sorted into CD133⁻ and CD133⁺ populations. SSEA1⁺ cells were excluded from both populations. (A) RNA was isolated from sorted cells, cDNA was prepared and RT-qPCR was conducted. Values represented are differences in Ct value (normalized by correcting for differences in Ct values of GAPDH), where a positive value indicates increased expression of a given transcript within the CD133⁺ population. (B) Freshly sorted i-SOX2-DAOY cells were placed into suspension culture in the absence or presence of Dox (1 µg/mL), as described in the Materials and Methods. Photomicrographs were taken after 5 days in culture.