Sublethal irradiation accelerates xenogeneic Graft-versus-Host-Disease (GvHD) in Hu-PBMC NSG mice.

<p>10⁷ freshly isolated PBMCs were injected intravenously via the tail vein into adult 6–12 week old NSG immunodeficient mice 24 hours post sub lethal irradiation (2.4 Gys). (A) Survival of irradiated Hu-PBMC NSG mice (MST = 14 days, <i>n</i> = 7, 2 PBMC donors). (B) The average weight is shown as a percentage of starting weight. Peripheral blood, spleen and bone marrow were harvested from irradiated Hu-PBMC NSG mice at the indicated time points and analysed for (C) human CD45 engraftment and (D) CD4:CD8 ratio. <b>Black bar represents human PBMC phenotype pre-injection.</b> Data represent the mean ±SEM and are compiled from 2 independent experiments (n = 7). (A) Log-rank Mantel-Cox Test, (B, D) unpaired t test, and (C) 2-way ANOVA, spleen vs blood (*,**) and spleen vs bone marrow (##).