Histone H1.5 is differentially distributed in fibroblasts and embryonic stem cells.

<p>(A) Venn diagram of significant peaks of H1.5 binding in H1 hESCs and IMR90 fibroblasts by ChIP-seq. (B) Pie chart of distribution of H1.5 relative to gene structure in H1 hESCs and IMR90 fibroblasts. (C) Venn diagram of number of H1.5 target genes in H1 hESCs and IMR90 fibroblasts. (D) Enrichment of HGNC gene family members in H1.5 target genes. Height of bars represents percentage of gene family members in all RefSeq genes (left) or H1.5 target genes (right). (E) Enrichment of clustered gene family members in H1.5 target genes. Height of bars represents percentage of clustered gene family members in all RefSeq genes (left) or H1.5 target genes (right). (F) Box plot of H1.5 enrichment levels of non-clustered gene family members (left) and clustered gene family members (right). (G) H1.5 enrichment block at the LCE/SPRR/S100A gene clusters. Each dot represents −Log10 of Poisson p-value of ChIPed DNA versus input DNA in a 100-bp window. Lines represent average values. LCE, SPRR and S100A genes are highlighted in orange, green and pink, respectively. (H) An H1.5 enrichment block in an intergenic region of chromosome 2. (I) Histogram of average significance of H1.5 enrichment (x-axis) in 5 kb windows versus the number of windows (y-axis) in H1 hESCs and IMR90 fibroblasts.