H1.5 distribution is established during cellular differentiation.

<p>Heat maps show the genome wide promoter distribution of H1.5 in (A) H1 hESCs and IMR90 fibroblasts, (B) HSF1 hESCs, neural progenitor cells (NPC), neural cells (Neu), and (C) primary keratinocytes (HK Ca−), calcium-treated keratinocytes (HK Ca+), and (D) primary human hepatocytes (Hepa). Each row represents the promoter of a gene in 500-bp intervals from −5.5 to +2.5 kb of the transcription start sites (TSS) which is indicated by the arrows. The gene promoters for all heat maps are ordered based on the highest to lowest level of H1.5 enrichment in fibroblasts. (E) Average H1.5 enrichment and expression of the epidermal (LCE, SPRR) and KRT gene clusters in HK Ca+ and IMR90 fibroblasts are shown as line charts and bar graphs, respectively. The relative position of genes in each cluster is illustrated schematically.