Knockdown of milton or Miro increases the levels of active PAR-1.

<p>(A) Western blots of eyes from flies expressing myc-tagged PAR-1 alone or flies co-expressing PAR-1 and milton RNAi^GD. Blots were probed with anti-phospho-T408 PAR-1 (P-PAR-1), anti-myc (PAR-1), or anti-tubulin. Tubulin was used as loading control. Mean ± SD, n = 5; *, p<0.05, Student's t-test. (B) Western blots of eyes from flies expressing myc-tagged PAR-1 or co-expressing PAR-1 and milton RNAi^TRiP. Blots were probed with anti-phospho-T408 PAR-1 (P-PAR-1), anti-myc (PAR-1), or anti-tubulin. Mean ± SD, n = 5; *, p<0.05, Student's t-test. (C) Western blots of eyes from flies expressing myc-tagged PAR-1 alone or flies co-expressing PAR-1 and Miro RNAi^KK. Blots were probed with anti-phospho-T408 PAR-1 (P-PAR-1), anti-myc (PAR-1), or anti-tubulin. Mean ± SD, n = 5; *, p<0.05, Student's t-test. (D) Western blots of eyes from flies expressing myc-tagged PAR-1 alone or flies co-expressing PAR-1 and luciferase RNAi. Blots were probed with anti-phospho-T408 PAR-1 (P-PAR-1), anti-myc (PAR-1), or anti-tubulin. No significant difference was found (mean ± SD, n = 5; p>0.05, Student's t-test). (E) Western blots of eyes from flies expressing myc-tagged PAR-1 T408A alone or flies co-expressing PAR-1 T408A and milton RNAi^GD. Blots were probed with anti-myc (PAR-1 T408A) or anti-tubulin. No significant difference was found (mean ± SD, n = 5; p>0.05, Student's t-test). Flies were 3 days-after-eclosion (day-old).