Crystal structure of HIV-1 CA^N in complex with CPSF6_313–327.

<p>(<b>A</b>) Crystal structure of the HIV-1 CA^N:CPSF6_313–327 complex. HIV-1 CA^N is shown as cartoon representation and CPSF6 as sticks. Secondary structure elements in HIV-1 CA^N are labeled. The electron density for the CypA-binding loop was poor, so this region is represented by a dashed line. (<b>B</b>) Close-up view of the HIV-1 CA^N:CPSF6_313–327 interface, showing HIV-1 CA^N as surface representation. The three CPSF6 residues that fill the centre of the channel in HIV-1 are indicated. (<b>C</b>) The CPSF6-binding interface on HIV-1 CA^N. Residues involved in binding to CPSF6_313–327 are labeled and shown in green. (<b>D</b>) Intramolecular interactions in CPSF6_313–327. Residues involved in intramolecular hydrogen bonding interactions are labeled and the interactions shown as dashed lines. The sequence of CPSF6_313–327 is shown for reference. (<b>E</b>) Stereo figure showing overview of the HIV-1 CA^N:CPSF6_313–327 interaction. The N- and C-termini of CPSF6_313–327 (labeled) project out of the binding channel in HIV-1 CA^N.