<p>A: The rubylated Cdc53 in Δ<i>csn5</i> extract was used as a substrate to test the deneddylation activity of Flag-purified mammalian complexes. Flag-S6CD, Flag-S6MPN, or empty vector were expressed in HeLa cells, isolated via the Flag beads, and eluted with the Flag peptide. The proteins were immunoblotted with indicated antibodies. Yeast cell extracts of wild type, Δ<i>rub1</i> and Δ<i>csn5</i> strains served as size markers for Cdc53 rubylated and un-rubylated forms. Asterisk indicates IgG background. B: Purified CSN complexes from yeast (ScCSN) or mammalian cells (CSN<sup>PS</sup>) were tested for the deneddylation activity on human Cul4a-Nedd8 conjugates in CSN-depleted HeLa cell extract. Δ<i>csn5</i> extract from yeast was used as a...
Background: The function of the fission yeast cullins Pcu1p and Pcu4p requires modification by the u...
Nedd8 is a ubiquitin-like modifier that is attached to the cullin components of E3 ubiquitin ligases...
<p>Human BRISC BRCA1-A, complexes are deubiquitinating enzymes. CSN is a deneddylase complex. The pr...
The CSN complex plays a key role in various cellular pathways: through a metalloprotease activity of...
<div><p>The COP9 signalosome (CSN) is a eukaryotic protein complex, which regulates a wide range of ...
<p>(<b>A</b>) <b>Studied protein fragments (brown)</b>. The MPN domain boundaries are indicated with...
The COP9 signalosome (CSN) is a regulator of the ubiquitin (Ub) proteasome system (UPS). In the UPS,...
<p>(A) <i>A. nidulans</i> DenA-CSN interactions in yeast-2-hybrid growth and β-galactosidase activit...
<p>Mice were challenged with DsRed <i>B</i>. <i>dermatitidis</i>, and lungs were harvested at 7 dpi....
<div><p>(A) Mutations in the glutamic acid (E56A) that positions the aqua ligand and in the proposed...
Nedd8 is a ubiquitin-like modifier that is attached to the cullin components of E3 ubiquitin ligases...
<p>(A) Quantitative analysis of repeated western blot experiments displayed the differences in DenA ...
<p><b>A:</b> Yeast-2-hybrid assay showing interaction of mouse Csn4 (EG-Csn4) with Csn6 (JG-S6-FL) a...
Deneddylases remove the ubiquitin-like protein Nedd8 from modified proteins. An increased deneddylas...
The COP9 signalosome (CSN) is a conserved protein complex with homologies to the lid subcomplex of t...
Background: The function of the fission yeast cullins Pcu1p and Pcu4p requires modification by the u...
Nedd8 is a ubiquitin-like modifier that is attached to the cullin components of E3 ubiquitin ligases...
<p>Human BRISC BRCA1-A, complexes are deubiquitinating enzymes. CSN is a deneddylase complex. The pr...
The CSN complex plays a key role in various cellular pathways: through a metalloprotease activity of...
<div><p>The COP9 signalosome (CSN) is a eukaryotic protein complex, which regulates a wide range of ...
<p>(<b>A</b>) <b>Studied protein fragments (brown)</b>. The MPN domain boundaries are indicated with...
The COP9 signalosome (CSN) is a regulator of the ubiquitin (Ub) proteasome system (UPS). In the UPS,...
<p>(A) <i>A. nidulans</i> DenA-CSN interactions in yeast-2-hybrid growth and β-galactosidase activit...
<p>Mice were challenged with DsRed <i>B</i>. <i>dermatitidis</i>, and lungs were harvested at 7 dpi....
<div><p>(A) Mutations in the glutamic acid (E56A) that positions the aqua ligand and in the proposed...
Nedd8 is a ubiquitin-like modifier that is attached to the cullin components of E3 ubiquitin ligases...
<p>(A) Quantitative analysis of repeated western blot experiments displayed the differences in DenA ...
<p><b>A:</b> Yeast-2-hybrid assay showing interaction of mouse Csn4 (EG-Csn4) with Csn6 (JG-S6-FL) a...
Deneddylases remove the ubiquitin-like protein Nedd8 from modified proteins. An increased deneddylas...
The COP9 signalosome (CSN) is a conserved protein complex with homologies to the lid subcomplex of t...
Background: The function of the fission yeast cullins Pcu1p and Pcu4p requires modification by the u...
Nedd8 is a ubiquitin-like modifier that is attached to the cullin components of E3 ubiquitin ligases...
<p>Human BRISC BRCA1-A, complexes are deubiquitinating enzymes. CSN is a deneddylase complex. The pr...