The structure of the <i>S. pombe</i> Swi1 protein.

<p>(A) The Swi1 polypeptide was divided into 9 putative functional sub-domains. Hatched boxes indicate the regions with amino acid sequences that are conserved throughout evolution. Swi1 contains the Timeless domain (22–279 aa), NLS (304–314 aa), the DDT domain (323–378 aa) and the Timeless-C domain (595–817 aa). h1, h2 and h3 in the DDT domain indicate alpha-helix regions. Swi1 also has stretches of acidic amino acids at 535–542, 858–867, and 916–924 aa regions. The four truncated versions of Swi1 constructed in this study are shown. aa, amino acid. (B) Multiple sequence alignment of DDT domains of various transcription factors, chromatin-remodeling proteins, human Tim1 (Timeless) and <i>S. pombe</i> Swi1. Conserved aromatic and hydrophobic residues are shown in red. The predicted helices are boxed. Asterisks indicate mutated amino acids in <i>swi1</i> mutants constructed in this study.