Effects of UV and FICZ on the EGFR signalling in NHEK.

<p><b>A</b>. Western blot detection of the phosphorylated forms of EGFR (P-EGFR) and ERK (P-ERK) in total lysates of untreated NHEK (-) and post-UV irradiation; <b>B</b>. Densitometry of the bands. *P<0.05 and ^§P<0.01 <i>versus</i> untreated controls (-). <b>C</b>. Western blots of nuclear non-phosphorylated (nEGFR) and phosphorylated EGFR (nP-EGFR) before and 20 min after exposure to UV. Histone 4 (H4) was used as a loading control; <b>D</b>. Densitometry of nEGFR and nP-EGFR bands. *P<0.05 <i>versus</i> untreated control (-). <b>E</b>. Western blot detection of the phosphorylated forms of EGFR (P-EGFR) and ERK (P-ERK) in total lysates of untreated NHEK (-) and after 30 min exposure to 0.1 or 1.0 µM FICZ. <b>F</b>. Densitometry of the bands. *P<0.05 <i>versus</i> untreated control (-). <b>G</b>. Western blots of nuclear non-phosphorylated (nEGFR) and phosphorylated EGFR (nP-EGFR) after 30 min exposure to 0.1 or 1.0 µM FICZ; <b>H</b>. Densitometry of nEGFR and nP-EGFR bands. *P<0.05 <i>versus</i> untreated controls.