Elevated FBXW8 diminishes specifically insoluble expanded ATXN2.

<p>(A) The protein levels of FBXW8 were significantly upregulated in the cerebellum of 18 months old CAG42 mice compared to wild-type (n = 9–11 mice/genotype). (B) Soluble levels of Q22-ATXN2 were unchanged after FBXW8 overexpression, while for Q74-ATXN2 a slight reduction was apparent. With respect to insoluble levels, Q22-ATXN2 was again not influenced by FBXW8 overexpression, while the Q74-ATXN2 reduction became significant. FBXW8 levels were significantly increased by its overexpression both in the soluble and insoluble fraction (n = 7).