Cell death is dependent on mtDNA.

<p>A, mitochondrial DNA and genomic DNA were evaluated by PCR from total DNA isolated from the indicated strains. Mitochondrial DNA content was assessed using the subunit I of cytochrome c oxidase gene (Cox1). Genomic DNA content was determined using the actin gene (Actin). B, Deletion of mtDNA protected <i>Fas2Δ/Δ</i> yeast cells from death induced by culture in serum. WT, <i>Fas2Δ/Δ</i> and <i>Fas2Δ/Δ</i> with partially deleted mtDNA (<i>Fas2-rho⁻</i>) were incubated with 50% serum for 24 and 48 h. Aliquots were serially diluted and WT yeast were spotted onto YPD whereas the <i>Fas2Δ/Δ</i> mutants were inoculated onto YPDT40. Experiments were performed twice with similar results. C, A proposed model of yeast cell sensitivity to glucose in the media in the setting of fatty acid inhibition.