P2 induces significant apoptosis and structural changes in the tight junction protein ZO-1 in in IEC-18 monolayers.
- Andrea D. Olivas (139745)
- Benjamin D. Shogan (139748)
- Vesta Valuckaite (139751)
- Alexander Zaborin (40806)
- Natalya Belogortseva (139756)
- Mark Musch (139758)
- Folker Meyer (11364)
- William L.Trimble (139763)
- Gary An (139765)
- Jack Gilbert (139767)
- Olga Zaborina (40802)
- John C. Alverdy (139768)
Publication date
August 2012
DOIAbstract
<p>(A) IEC-18 cells infected with P1 and P2 for 3 hrs were analyzed for apoptosis and necrosis with Apoptic&Necrotic&Healthy Cells Quantification kit (Biotium, Inc.) using fluorescence microscope Axiovert 35 (Zeiss,Germany). FITC-Annexin V (apoptotic cells, green), EtD-III (necrotic cells, red). (B) Staining of nuclei with Hoechst 33342. (C) IEC-18 monolayers treated with antibody to ZO-1.</p
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