miR-127 is modulated in response to Hypoxia/Reoxygenation <i>in vitro</i> and during Ischemia/Reperfusión <i>in vivo</i>.

<p>(A) rno-miR-127 modulation in rat proximal tubule cells NRK-52E (Upper panel) and human proximal tubule cells HK-2 (Lower panel) submitted to hypoxia/reoxygenation (H/R) protocol. microRNA detection was performed by quantitative PCR using specific assays for miR-127 and RNU6B, which was employed as housekeeping control. Fold values were obtained by ΔΔCt method using Normoxia (Nx) as basal condition. Data are presented as mean±s.e.m. of five independent experiments in each panel. Asterisks indicate statistical significance comparing each sample with Normoxia (P<0.05). (B) rno-miR-127 modulation in rat kidney in response to ischemia/reperfusion (I/R). miRNA expression was estimated by quantitative PCR using specific taqman assays. Ribosomic RNA 5s was used as housekeeping gene and fold values were obtained comparing each group to Sham operated animals. Data are presented as mean±s.e.m. of at least five animals per condition. Asterisks indicate statistical significance (P<0.05) comparing each experimental group to Sham animals. (Nx: Normoxia; CC: Medium change control; Hyp CM: hypoxia in complete medium; Hyp MM: hypoxia in minimum medium; R-1h: Hypoxia in minimum medium and 1 Hour reoxygenation; R-3h: Hypoxia in minimum medium and 3 hours of reoxygenation; R-6h: Hypoxia in minimum medium and 6 hours of reoxygenation; R-24h Hypoxia in minimum medium and 24 hours of reoxygenation; I/R-24h: ischemia and 24 hours of reperfusion; I/R 3D: ischemia and 3 days of reperfusion; I/R 5D: ischemia and 5 days of reperfusion; I/R 7D: ischemia and 7 days of reperfusion).