Resveratrol inhibits OVA plus CT-induced Th1/2 cell differentiation <i>in vitro</i>.

<p>DO11.10 mice-derived splenocytes were un-stimulated or stimulated with 300 µg/ml OVA plus 12 pM CT and were cultured in the presence or absence of 10 or 30 µM resveratrol for the indicated times. Then the analysis described below was performed. A. After 3 day-culture, RNA samples were extracted from the cells and the cDNA samples were synthesized using reverse transcriptase system. Quantitative real-time PCR analysis was then done for T-bet, GATA3, and Foxp3 mRNAs. Relative expression levels are shown (n = 3 per group). B. After 3 day-culture, the supernatants were collected and IFN-ã, IL-4, and IL-13 concentrations were measured by ELISA (n = 9 per group). C. After 3 day-culture, the cells were subjected to a WST assay for evaluation of the cell viability (n = 4 per group). Values represent the mean ± SD. *P<0.05 in comparison to the corresponding controls. (ND: non-detected).