miR-127 regulates trafficking in rat proximal tubule cells in response to H/R.

<p>(A)Non receptor-mediated endocytosis assays in NRK-52E cells transfected with pre-miR-127, anti-miR-127 and their respective scramble control. Dextran-FITC of 70 KDa was added to culture medium and nuclei were stained with DAPI. Representative confocal images from five independent experiments are shown. (B) Quantification of previous images expressed as green signal surface/blue signal surface ratio, each signal estimated in pixels². (Nx: Normoxia; CC: Medium change control; Hyp MM: hypoxia in minimum medium; R-6h: Hypoxia in minimum medium and 6 hours of reoxygenation).