shRNA MGAT1 suppresses N-glycan branching.

<p><b>A</b>) HeLa cells were infected with lentiviral vectors targeting MGAT1(shRNA1 or shRNA2) or the control shRNA sequences. Stable cell populations were selected by the addition of puromycin (1 µg/mL). <b>A</b>)MGAT1 mRNA were measured by qRT-PCR, <b>B</b>) MGAT1 enzyme activity, <b>C</b>) L-PHA reactive surface N-glycans by Array scan microscope, <b>D</b>) Proliferation over 4 days Data represent the mean ± SD relative expression of mRNA relative to control sequence (n = 3 independent experiments performed in triplicate).