Figure 4

<p>(A) RT-PCR for 18S RNA in culture medium (CM) from BMSC controls and culture medium treated with RNase (CM+RNAse). (B) BMSC viability after treatment with Trypsin and RNase by Trypan blue extrusion. (C) The light micrographs of the kidney sections stained with hematoxylin eosin and with immunochemistry for caspase 3 and KI67. (D) A graphic representation showing the quantitative analysis of the control rat micrographs (CTL₁₅; CTL₁₅+BMSC₁₀). The histology of the kidney tissue from rats treated with G for 15 days (G₁₅). Some groups presented with massive tubular necrosis and unstained nuclei (G₁₅, G₁₅+RNAse+CM₁₅), while other groups presented with intensely stained nuclei (G₁₅+BMSC₁₀, G₁₅+CM₁₀). Data are expressed as the mean ± S.E.M. (* p<0.05) vs. CTL15d, (# p<0.05) vs. G15.