L1 arrest is a starvation response depending on metabolic rates.

<p>A L1s arrested at low temperature live longer than those arrested at high temperature. Eggs were collected and hatched without food and L1s were maintained at the indicated temperatures <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044720#pone.0044720-You1" target="_blank">[26]</a>. For all L1 longevity assays, each sample contained approximately 10 worms/µl and each plate was plated with approximately 150–200 worms. Every experiment was repeated at least three times with triplicates (see Materials and Methods). B LT 50 (50% lethality) for worms that arrested at 15°C, 20°C and 25°C. C Life spans of arrested L1s of <i>daf-7</i> and <i>daf-3</i> mutants tested at 25°C. D Rates of oxygen consumption in wild type, <i>daf-2</i> and <i>daf-16</i> mutants (**<i>p<0.01</i>, by two way ANOVA). E Rates of oxygen consumption in wild type and <i>daf-18</i> mutants <i>(*p<0.05</i>, by two way ANOVA).