Validation of exemplary transcription factors.

<p>NIH-3T3 fibroblasts were infected with MCMV at an MOI of 10 for the indicated time points and lysates were prepared for western blot analysis (<b>A</b>), for immune staining (<b>B</b>) or luciferase assay (<b>C</b>). Western blot analysis was performed on samples prepared from uninfected and infected NIH-3T3 cells probed for RelA and IkBα (<b>A</b>). GAPDH was probed as loading control. For the immunofluorescence staining (<b>B</b>) cells were fixed and stained with the indicated antibodies; white circle indicating nucleus, nuclear dimensions were acquired by DAPI staining and its outline was overlaid into the shown channels; green, RelA; red, viral IE1. For the luciferase assays cells were transfected with a c-Myc-reporter construct (<b>C</b>) and infected 48 hours post transfection with MCMV at an MOI of 10. At the indicated times post infection, Firefly-Luciferase measurements were performed in triplicates. Shown is the mean +/− SD of a representative of three experiment; mpi = minutes post infection, hpi = hours post infection.