KAHsp40 associates with knobs on the infected erythrocyte membrane.

<p>(A-B) IFA reveals that KAHsp40 and KAHRP co-localize in the erythrocyte periphery near the erythrocyte plasma membrane. White arrows indicate the discrete foci in which they co-localize. (C) Co-IP of KAHsp40 from total infected erythrocytes reveals two interactors, of about 66 kDa and 150 kDa. (D) Reciprocal co-IP immunoprecipitates analyzed on a 5–15% gradient gel reveals that 66 kDa interactor of KAHsp40 is KAHRP. (E) Western blot of KAHRP co-IP immunoprecipitate shows the presence of KAHsp40. (F-G) IFA reveals that KAHsp40 and PfEMP1 co-localize with each other on the erythrocyte plasma membrane indicating the close association of KAHsp40 with knobs. (H-I) IFA reveals that KAHsp40 and PfEMP3 co-localize with each other on the erythrocyte plasma membrane indicating the close association of KAHsp40 with knobs. (J) Western blot of KAHsp40 co-IP immunoprecipitate shows the presence of PfEMP3. The images shown have been taken at the trophozoite stage.