<i>Rgs4</i> is responsive to hypoxia and hypoxia-mimetic chemicals.

<p>(A) Northern blot of total RNA from parental PC-12 cells with or without 16 hours of hypoxic treatment, and the TetON-Control, TetON-HIF1α and TetON-HIF-2α PC-12 cell lines with or without 16 hours of doxycycline treatment (Dox). Blot shown is representative of either three (<i>Rgs4</i> with doxycycline treatments), two (<i>Rgs4</i> with hypoxia, <i>Vegf</i> with doxycycline) or one (<i>Vegf</i> with hypoxia) independent experiments. (B) SK-N-SH cells were treated with normoxia, hypoxia, 0.1% DMSO (vehicle), 1 mM DMOG or 100 µM DP for 16 hours before target gene analysis by qRT-PCR. Mean fold change with standard deviation for three independent experiments is shown. (C) PC-12 and (D) SK-N-BE(2)C cell lines were tested in two independent experiments as in 1(B). Representative experiments (from n = 3) are shown. (E) SK-N-BE(2)C and HUVEC cell cultures were treated with 0 (normoxia), 3 and 16 hours of hypoxia before quantification of <i>Rgs4</i>, <i>Rgs5</i> and <i>Vegf</i> mRNA levels relative to <i>Polr2a</i> using qRT-PCR, normalised to normoxic levels. Mean fold change with standard deviation for three independent experiments is shown. Asterisks indicate that the normoxic control lies outside of the 99% confidence interval of relative mean target gene expression.