Comparison of effect of 4a and Levamisole on progression of solid tumor in mice.

<p>Solid tumor was induced in Swiss albino mice by injecting EAC cells. Six doses of <b>4a</b> and Levamisole (20 mg/kg) each administered to tumor bearing mice on every alternate day from 12^th day of EAC cell injection. <b>A.</b> Effect of <b>4a</b> and Levamisole on tumor progression at different time points. Data shown is based on two independent batches of experiments containing four animals each. Error bars indicate SD from independent experiments. <b>B.</b> Kaplan–Meier survival curves of mice treated with <b>4a</b>. Out of 24 tumor induced Swiss Albino animals, 12 were treated with <b>4a</b> (20 mg/kg) and survival graph was plotted, Log-rank statistical test showed P<0.005 (**). In control case, median survival time was found to be 59 days and in case of 4a treated it is undefined (value showed up to 250 days). <b>C.</b> Gross appearance of <b>4a</b> treated and untreated tumor mice and their selected organs at 25^th day of treatment. <b>a.</b> mouse with no tumor, <b>b.</b> mouse bearing tumor, <b>c.</b> tumor bearing mouse after treatment with <b>4a</b>, <b>d</b>. thigh tissue of normal mouse, <b>e.</b> tumor, <b>f.</b> thigh tissue of a treated mouse, <b>g.</b> liver from normal mouse, <b>h</b>. liver of a tumor mouse, <b>i.</b> liver from a <b>4a</b> treated mouse, <b>j</b>. spleen of a normal mouse, <b>k.</b> spleen of a mouse with tumor, <b>l.</b> spleen of a treated mouse.