Determination of intracellular ROS production in CEM and REH cells following treatment with 4a.

<p><b>A, B.</b> CEM (A) and REH (B) cells treated with <b>4a</b> (5 µM and 10 µM, respectively) for different time points were used for testing the formation of intracellular ROS by flow cytometry analysis. The concentration selected for the study was based on their respective IC₅₀ values. H₂O₂ treated cells were used as positive control while cells alone were used as negative control. DMSO treated cells were used as vehicle control. Cell population showing ROS was shown along with standard error mean (n = 2).