Analysis of expression patterns of major ripening genes in different zones of banana fruit.

<p><b>a–e</b> Semi-quantitative RT-PCR analysis was carried to detect the changes of transcript level of <i>MA-SPS, MA-ACS1</i>, <i>MA-ACO1</i>, <i>MA-EXP</i> and <i>MA-LEC</i> in A and B zones of preclimacteric (lanes 1 and 2), climacteric (lanes 3 and 4) and postclimacteric (lanes 5 and 6) banana fruit. <b>g–k</b> Detection of transcript levels of the selected ripening genes from peripheral and central regions of C, D and E zones of preclimacteric (0 DAH) banana fruit pulp. <b>m–q</b> Transcript abundance of the indicated ripening genes in the peripheral and central regions of C, D and E zones of climacteric (88 DAH) banana fruit pulp. <b>s–w</b> Changes in transcript levels of the ripening genes in peripheral and central regions of C, D and E zones of postclimacteric (92 DAH) banana fruit pulp. Figures f, l, r and x represent relative message levels of <i>MA-Actin</i> measured under the corresponding conditions as internal control. Representative images from at least three independent experiments are shown.