Exemplar segmented fluorescence intensity images, fitted fluorescence decay and fluorophore emission spectra.
- Rakesh Patalay (136903)
- Clifford Talbot (136905)
- Yuriy Alexandrov (136907)
- Martin O. Lenz (136909)
- Sunil Kumar (102321)
- Sean Warren (136911)
- Ian Munro (136913)
- Mark A. A. Neil (136915)
- Karsten König (136916)
- Paul M. W. French (122489)
- Anthony Chu (136919)
- Gordon W. H. Stamp (136921)
- Chris Dunsby (122481)
Publication date
September 2012
DOIAbstract
<p>(a) Total fluorescence intensity image, same image with (b) manually and (c) automatically defined cellular regions of interest overlayed. (d) Top – exemplar fluorescence decay from one region of interest (black), biexponential fit to data (green) and instrument response function (blue). (d) Bottom – normalized residuals. (e) The emission spectra from endogenous fluorophores plotted in relation to the four spectral detection channels.</p
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