Effect of suspension culture and TSA treatment on histone acetylation of hESC-CM colonies.

<p>Western blot analyses show increased histone H3 acetylation (AcH3) levels in 35-day-old hESC-CMs (A) following 14 days of suspension culture (Sus) or (B) following treatment with 100 nM TSA for 48 hrs under adhesive (Ad) culture conditions (DMSO treatment had no effect on AcH3 levels). (C) Results from western blot analyses comparing Sus to Ad cultures (n = 9) and TSA to DMSO treatments (n = 13) show increased AcH3 levels in hESC-CMs. (D) Results of qRT-PCR analysis showing that TSA increases cardiac gene expression in hESC-CMs for at least 48 hrs after treatment. The corresponding values for human adult hearts (AH) are shown for each sample. Each graph displays the mean and standard deviation (SD) of three independent experiments. *, p<0.05; **, p<0.01.