Double immunofluorescence labeling of ED1 (red) and TSPO (green) displayed as a two-channel image.

<p>The images demonstrated temporal alterations of TSPO expression in macrophages at 2 h (D–F), 6 h (G–I) and 24 h (J–L) after LPS inducement, and in the control (A–C). Arrows indicate examples of cells doubly positive for ED1 and TSPO. (A–C) TSPO expression was seen in a few macrophages. (D–F) Several macrophages showing TSPO immunoreactivity were observed in the alveolar spaces. (G–I, J–L) Numbers of macrophages with TSPO increased gradually over time. Scale bar: 20 µm.