RomR interacts directly with MglA and MglB.

<p>(A) RomR interacts with His₆-MglB and MglA-His₆. WT <i>M. xanthus</i> cell extract was applied to a Ni⁺⁺-NTA-agarose column with or without bound His₆-MglB (left) and with or without MglA-His₆ (right). Eluted proteins were separated by SDS-PAGE and visualized in immunoblots with α-RomR (upper panels) or by Coomassie Brilliant Blue R-250 staining (lower panels). Positions of His₆-MglB, MglA-His₆ and RomR including their calculated molecular masses are indicated. Migration of molecular weight markers in kDa is indicated on the left. (B) RomR interacts directly with MglA. Purified His₆-RomR was applied to a glutathione-agarose column with bound GST-MglA (left) or with bound GST (right). Shown are proteins from the last wash fraction before elution (W) and from the elution (E). Eluted proteins were separated by SDS-PAGE and visualized in immunoblots with α-GST (upper panels) and α-RomR (lower panels). GST- MglA and His₆-RomR including their calculated molecular masses are indicated. Migration of molecular weight markers in kDa is indicated on the left. (C) RomR interacts directly with MglB. Purified His₆-MglB was applied to an amylose-agarose column with bound MalE-RomR (left) or with bound MalE (right). Shown are proteins from the last wash fraction before elution (W) and from the elution (E). Eluted proteins were separated by SDS-PAGE and visualized in immunoblots with α-MalE (upper panels) and α-MglB (lower panels). MalE-RomR, His₆-MglB and MalE including their calculated molecular masses are indicated. Migration of molecular weight markers in kDa is indicated on the left.