Expression and activation of EGFR and Met in mammary epithelial cells. A.

<p>Western-blot analysis of EGFR and Met expression in human MCF-10A, murine NMuMG and bovine BME-UV mammary epithelial cells. Mouse liver extract was used as a positive control for both Met and EGFR. <b>B.</b> Western-blot analysis of EGFR, Met, ERK1/2 and AKT phosphorylation in MCF-10A and NMuMG cells cultivated in serum-starving medium and treated with EGF or HGF (20 ng/ml) for 10, 30 and 60 min. EGFR, Met, ERK1/2 and AKT were used to show comparable loadings between the lanes.