Fatty acid analysis of <i>C. reinhardtii</i> strains expressing thioesterases.

<p>Fatty acid composition of Cr strains was determined by GC/MS analysis and comparison to authentic standards. Peak areas were integrated and compared to an external standard for quantification. Bar graphs denote abundances of (<i>A</i>) Myristic acid (14:0), (<i>B</i>) Palmitic acid (16:0), and (<i>C</i>) Oleic acid (18:1), and labels on the Y-axis correspond to the percentages of these fatty acids of the total fatty acid content. (<i>D</i>) Full GC/MS chromatograms of Cr strains expressing CrTE (red), UcTE (Blue) and wildtype CrTE (black). Three separate cultures of each strain were analyzed for fatty acid content and composition, and data were recorded and averaged with a mean deviation of 7% in each experiment. Statistical analyses were performed using SPSS (v13.0), and for all data analysis, a <i>p</i>-value<0.5 was considered statistically significant.