Analysis of hybrid F₁ plants co-transgenic for both integrase and target sequence.

<p>The results of the PCR analyses are summarized. The copy number of the target sequence was estimated by DNA gel blot analysis.</p><p>Independent co-transgenic hybrid plants carrying both the HW511 and either the ICH13130 or the ICH14313 loci, or a recombinant derivate were included in the analysis. Descendants that lost the target locus or the integrase locus or both due to segregation in meiosis of T₀ plants are not listed in the table.</p><p>“+”/“−“ indicates the presence/absence of recombination.</p>*<p><b>¹</b> Primary transformants (T₀) that were hemizygous for the target locus were crossed with the integrase lines.</p>*<p><b>²</b> PCR using primers <i>RecFw</i> and <i>RecRev</i> resulted in the production of a 400 bp fragment containing <i>attR</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045353#pone-0045353-g001" target="_blank">Fig. 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045353#pone-0045353-g002" target="_blank">2</a>). The number of individuals that contained a recombination event and a non-recombinant locus (which was identified by PCR-amplification of GFP with the primers <i>gfpFw and gfpRev</i> and/or by the amplification of a 1.5 kb fragment using primers <i>RecFw</i> and <i>RecRev</i>) is indicated in <i>brackets.</i></p>*<p><b>³</b> PCR using primers <i>RecFw</i> and <i>RecRev</i> resulted in the production of a 1.5 kb fragment containing <i>GFP</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045353#pone-0045353-g001" target="_blank">Fig. 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045353#pone-0045353-g002" target="_blank">2</a>).