IFN-γ does not accelerate necrotic cell death in <i>Toxoplasma</i>-infected SkMCs.

<p>Differentiated C2C12 SkMCs (A,B) and NIH/3T3 fibroblasts (C,D) were either infected with <i>T. gondii</i> (NTE strain, parasite-host cell ratio 2∶1; B,D), or were mock-infected (A,C). At the time of infection, cells were stimulated with IFN-γ alone (open squares) or combined with TNF (open triangles), or were left non-stimulated (closed circles). Lactate dehydrogenase (LDH) activity was monitored in culture supernatants at different time points of infection. Maximal LDH activity of each sample was determined in parallel by complete cell lysis. LDH activities in the supernatants of experimental samples are expressed as percentages of the maximal activity; data represents means ± S.E.M. from three independent experiments.