T-DNA constructs used to generate the USDA-ARS-WRRC T-DNA collection.

<p><b>A.</b> Illustrations of the T-DNA regions of pOL001 and the seven new constructs used to generate the majority of the population are shown. White boxes: T-DNA left border (LB or 2LB) and right border sequences (RB). Light gray boxes: <i>Z. mays</i> ubiquitin promoter with intron (ZmUbi) and cauliflower mosaic virus 35S promoter (CaMV 35S). Dark grey boxes: nos terminator sequences (T_nos). Red boxes: cauliflower mosaic virus 35S transcriptional enhancer sequences (4× CaMV35S enhancer). Black arrows: hygromycin phosphotransferase selection gene (<i>HptII</i>) or phosphinothricin acetyltransferase selection gene (<i>BAR</i>). Blue arrows and boxes: ß-glucuronidase reporter genes (<i>GUS</i> and <i>GUSPlus</i>) with a rice tubulin intron containing splice donor and acceptor sites (I2). Green arrow and box: green fluorescent protein reporter gene (<i>sGFP</i>) with a rice tubulin intron containing splice donor and acceptor sites (I3). <b>B.</b> Pie chart showing the composition of the T-DNA population. The yellow represents lines made with the insertional mutagenesis vectors including pOL001, pJJH, pJJB, pJJ2LB, and pJJB2LB. The blue represents lines made with the gene trap vectors pJJ2LBP and pJJ2LBP2, and the red represents lines made with the activation tagging vector pJJ2LBA.