Protective effect of IRF3 on the MAVS protein.

<p>(A) A549 cells were transfected with polyI:C and incubated for up to 8 h. (B) Following IRF3 knockdown, A549 cells were transfected with polyI:C. Cell extracts were subjected to either SDS- (A and B) or native PAGE (A) and then blotted with anti-IRF3, anti-MAVS or anti-actin antibodies, as indicated. The results are representative of three independent experiments.