Plotted results of ANIstt (average nucleotide identity of <i>SMc00019-truA-thrA</i>) versus ANIm (genomic ANI).

<p>460 intra-genus pairwise comparisons in <i>Bradyrhizobium</i> (190), <i>Mesorhizobium</i> (3), <i>Sinorhizobium</i> (91), <i>Rhizobium</i> (10), <i>Agrobacterium</i> (6), <i>Cupriavidus</i> (10), <i>Methylobacterium</i> (28), <i>Ralstonia</i> (10), <i>Rhodopseudomonas</i> (21) and <i>Burkholderia</i> (91). 7/460 points lie between the interspecies region (ANIm <93% and ANIstt <94%) and the intraspecies region (ANIm >95% and ANIstt >96%). The thick and fine solid line(s) depicted the quadratic curve (the best curve to fit the data) and the individual confidence intervals (95%). The quadratic equation and R square are shown.